Catalog Number - 68508-05P-4-3M

Cogent RP C8™ Wide Pore HPLC Columns are designed for reversed-phase separations of larger molecules that require enhanced pore accessibility and efficient mass transfer. The stationary phase consists of a traditional octyl (C8) bonded ligand attached to ultra-high purity silica and is fully double end capped to minimize secondary interactions while delivering consistent retention and excellent peak shape. The 300 Å wide pore structure allows proteins, peptides, and other larger analytes to access more of the bonded surface area, making this phase well suited for biomolecule separations and other high molecular weight compounds.

68508-05P-4-3M is packed with 5 µm RP C8™ Wide Pore particles featuring a 300 Å pore size in a 4.0 mm ID x 50 mm length format. The short column length provides rapid analyses and reduced solvent consumption while maintaining excellent chromatographic performance. The wide pore architecture is particularly advantageous for proteins, peptides, and long-chain fatty acids that may experience restricted pore access in conventional 100 Å media. The RP C8™ chemistry provides lower hydrophobic retention than a comparable C18 phase while maintaining excellent selectivity for hydrophobic and moderately non-polar compounds.

The 4.0 mm ID format offers excellent compatibility with conventional analytical HPLC systems and is commonly used for pharmaceutical, biopharmaceutical, and method development applications. The combination of low backpressure, fast analysis times, and improved accessibility for larger molecules makes this column a practical choice for both routine and research laboratory workflows.

Key Benefits

• RP C8™ Wide Pore stationary phase

• Octyl (C8) bonded phase

• Fully double end capped

• Ultra-high purity silica support

• 300 Å wide pore design

• Suitable for proteins and peptides

• Lower retention than C18 phases

• Fast 50 mm column length

• Low backpressure with 5 µm particles

• Compatible with standard HPLC systems

Typical Applications

• Reversed-phase HPLC

• Protein analysis

• Peptide analysis

• Biomolecule separations

• Long-chain fatty acid analysis

• Method development

• Pharmaceutical analysis

• Biopharmaceutical analysis

• Rapid screening applications

• Routine analytical testing

Specifications

• Product Type: HPLC Column

• Stationary Phase: RP C8™ Wide Pore

• Chromatography Mode: Reversed Phase

• Bonded Phase: Octyl (C8)

• Endcapping: Fully Double End Capped

• Particle Size: 5 µm

• Pore Size: 300 Å

• Column Dimensions: 4.0 mm ID x 50 mm

• Recommended Flow Rate: 0.8-1.5 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Special Order Item Information

• Please review the Special Order Item Information for complete details.

• For pricing confirmation, availability, lead times, or application assistance, please contact MICROSOLV Customer Service.

Important Notes

• Designed for larger molecule reversed-phase separations

• 300 Å pores enhance accessibility for proteins, peptides, and other biomolecules

• Provides lower retention than comparable C18 columns

• Suitable for hydrophobic and moderately non-polar compounds

• Excellent choice for long-chain fatty acids, peptides, and selected proteins

• 50 mm length helps reduce analysis times and solvent consumption

• 5 µm particles provide low backpressure and broad instrument compatibility

• Compatible with standard HPLC systems operating at conventional flow rates

• Manufactured using ultra-high purity silica and fully double end capped bonding technology

• This is a Special Order Item